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Related Concept Videos

Subcellular Fractionation01:32

Subcellular Fractionation

The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
Differential Centrifugation
Differential centrifugation is...
Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
Centrifugation01:05

Centrifugation

Centrifugation is a separation technique based on differences in density or size. It is commonly used to separate solids from aqueous interferents. During centrifugation, the sample is placed in centrifugation tubes and spun at high angular velocity, which allows centrifugal force to act differentially on the different densities or masses of the components. After spinning, the supernatant liquid is decanted. Depending on the specific application, either the pellet or the supernatant is retained...

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Resting (basal) secretion of proteins is provided by the minor regulated and constitutive-like pathways and not granule exocytosis in parotid acinar cells.

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Tyrosine phosphorylation of selected secretory carrier membrane proteins, SCAMP1 and SCAMP3, and association with the EGF receptor.

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Protein secretion by rat parotid acinar cells. Pathways and regulation.

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Related Experiment Video

Updated: Jul 7, 2026

Cell Fractionation of U937 Cells in the Absence of High-speed Centrifugation
06:53

Cell Fractionation of U937 Cells in the Absence of High-speed Centrifugation

Published on: January 18, 2019

Overview of cell fractionation.

J D Castle1

  • 1University of Virginia, Charlottesville, Virginia, USA.

Current Protocols in Cell Biology
|January 30, 2008
PubMed
Summary

Cell fractionation isolates cellular components for analysis and in vitro studies. This overview covers centrifugation principles, equipment, media, and optimization for effective cell biology research.

Area of Science:

  • Cell Biology
  • Biochemistry

Background:

  • Cell fractionation is crucial for understanding cellular components.
  • It enables analysis of subcellular structures and their functions.
  • Essential for preparing samples for in vitro reconstitution experiments.

Purpose of the Study:

  • To provide an overview of cell fractionation techniques.
  • To discuss the fundamental principles of centrifugation in cell biology.
  • To guide researchers on optimizing fractionation procedures.

Main Methods:

  • Centrifugation principles and instrumentation.
  • Selection criteria for appropriate media.
  • Methods for evaluating fractionation efficiency.
  • Strategies for procedure optimization.

More Related Videos

Cell Fractionation of U937 Cells by Isopycnic Density Gradient Purification
10:19

Cell Fractionation of U937 Cells by Isopycnic Density Gradient Purification

Published on: August 12, 2021

Subcellular Fractionation from Fresh and Frozen Gastrointestinal Specimens
10:07

Subcellular Fractionation from Fresh and Frozen Gastrointestinal Specimens

Published on: July 15, 2018

Related Experiment Videos

Last Updated: Jul 7, 2026

Cell Fractionation of U937 Cells in the Absence of High-speed Centrifugation
06:53

Cell Fractionation of U937 Cells in the Absence of High-speed Centrifugation

Published on: January 18, 2019

Cell Fractionation of U937 Cells by Isopycnic Density Gradient Purification
10:19

Cell Fractionation of U937 Cells by Isopycnic Density Gradient Purification

Published on: August 12, 2021

Subcellular Fractionation from Fresh and Frozen Gastrointestinal Specimens
10:07

Subcellular Fractionation from Fresh and Frozen Gastrointestinal Specimens

Published on: July 15, 2018

Main Results:

  • The abstract does not contain specific results.
  • It outlines key considerations for successful cell fractionation.

Conclusions:

  • Effective cell fractionation requires understanding centrifugation basics.
  • Careful selection of media and optimization are key to reliable results.
  • This method is vital for advancing cell biology research.