Related Experiment Video
Updated: Jul 7, 2026

Isolation of Intact Mitochondria from Skeletal Muscle by Differential Centrifugation for High-resolution Respirometry Measurements
Published on: March 8, 2017
Isolation of mitochondria from tissues and cells by differential centrifugation
1Liverpool John Moores University, Liverpool, United Kingdom.
Abstract:
Differential centrifugation is used to prepare a "heavy" mitochondrial fraction from liver. These mitochondria are relatively pure, highly coupled, and suitable for respiratory studies. This unit presents protocols for isolation of beef heart mitochondria (also suitable for respiratory studies), skeletal muscle mitochondria, and mitochondria from cultured cells can also be isolated from homogenates by differential centrifugation. Differential centrifugation, which separates cellular organelles based on sedimentation velocity, is a rapid method for preparing mitochondria for metabolic studies.
Related Concept Videos
Subcellular Fractionation
Differential Centrifugation
Differential centrifugation is...
Overview Of Cell Separation And Isolation
Centrifugation

