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Overview of subcellular fractionation procedures for the yeast Saccharomyces cerevisiae

S E Rieder1, S D Emr

  • 1University of California, San Diego, La Jolla, California, USA.

Current Protocols in Cell Biology
|January 30, 2008
PubMed

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Related Concept Videos

Subcellular Fractionation01:32

Subcellular Fractionation

The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
Differential Centrifugation
Differential centrifugation is...