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Visualization of Endoplasmic Reticulum Subdomains in Cultured Cells
Published on: February 18, 2014
Fluorescent staining of subcellular organelles: ER, Golgi complex, and mitochondria
M Terasaki1, L Loew, J Lippincott-Schwartz
1University of Connecticut Health Center, Farmington, Connecticut, USA.
Current Protocols in Cell Biology
|January 30, 2008
Abstract:
The ability to distinguish and identify specific subcellular compartments is essential to understanding organelle function, biogenesis, and maintenance within cells and to defining protein trafficking pathways. Fluorescent dyes and/or fluorescently labeled lipid derivatives can be used to identify ER, Golgi complex, and mitochondria. Specific conditions for labeling each of these compartments are described.
