Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Two-dimensional Gel Electrophoresis

Two-dimensional Gel Electrophoresis

Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as cells...

Electrophoresis: Overview

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...

Capillary Electrophoresis: Applications

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...

SDS-PAGE

SDS-PAGE

Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
A variation of gel electrophoresis, termed polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact proteins...

DNA Agarose Gel Electrophoresis

DNA Agarose Gel Electrophoresis

Agarose gel electrophoresis is a laboratory technique commonly used to separate DNA fragments by size. However, it can also be used to isolate and purify DNA fragments using a gel extraction protocol.
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...

Capillary Electrophoresis: Instrumentation

Capillary Electrophoresis: Instrumentation

Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Quantitation of DNA and RNA with absorption and fluorescence spectroscopy.

Current protocols in immunology·2008

Same author

Quantitation of DNA and RNA with absorption and fluorescence spectroscopy.

Current protocols in neuroscience·2008

Same author

One-dimensional SDS gel electrophoresis of proteins.

Current protocols in protein science·2008

Same author

One-dimensional electrophoresis using nondenaturing conditions.

Current protocols in protein science·2008

Same author

One-dimensional electrophoresis using nondenaturing conditions.

Current protocols in molecular biology·2008

Same author

Quantification of DNA and RNA with absorption and fluorescence spectroscopy.

Current protocols in cell biology·2008

Search research articles

Related Experiment Video

Updated: Jul 7, 2026

Use of Two Dimensional Semi-denaturing Detergent Agarose Gel Electrophoresis to Confirm Size Heterogeneity of Amyloid or Amyloid-like Fibers

Use of Two Dimensional Semi-denaturing Detergent Agarose Gel Electrophoresis to Confirm Size Heterogeneity of Amyloid or Amyloid-like Fibers

Published on: April 26, 2018

One-dimensional electrophoresis using nondenaturing conditions.

S R Gallagher¹

¹Motorola Corporation BioChip Systems, Tempe, Arizona, USA.

Current Protocols in Cell Biology

|January 30, 2008

Summary

This unit presents two nondenaturing gel electrophoresis protocols for analyzing protein size, subunit structure, and separation. Native gel electrophoresis is a versatile technique for protein analysis.

Area of Science:

Biochemistry
Molecular Biology
Proteomics

Background:

Native gel electrophoresis separates proteins based on size, shape, and charge without denaturation.
Understanding protein structure and interactions is crucial in molecular biology.

Purpose of the Study:

To present two protocols for nondenaturing or native gel electrophoresis.
To offer a technique for analyzing native protein size, subunit structure, and optimal separation.

Main Methods:

Nondenaturing polyacrylamide gel electrophoresis (PAGE).
Continuous PAGE for broad pH separations (cationic and anionic).
Discontinuous PAGE for negatively charged proteins at neutral pH.

Main Results:

More Related Videos

Analysis of Thylakoid Membrane Protein Complexes by Blue Native Gel Electrophoresis

Analysis of Thylakoid Membrane Protein Complexes by Blue Native Gel Electrophoresis

Published on: September 28, 2018

Denaturing Gradient Gel Electrophoresis (DGGE)

Denaturing Gradient Gel Electrophoresis (DGGE)

Published on: February 25, 2007

Related Experiment Videos

Last Updated: Jul 7, 2026

Use of Two Dimensional Semi-denaturing Detergent Agarose Gel Electrophoresis to Confirm Size Heterogeneity of Amyloid or Amyloid-like Fibers

Use of Two Dimensional Semi-denaturing Detergent Agarose Gel Electrophoresis to Confirm Size Heterogeneity of Amyloid or Amyloid-like Fibers

Published on: April 26, 2018

Analysis of Thylakoid Membrane Protein Complexes by Blue Native Gel Electrophoresis

Analysis of Thylakoid Membrane Protein Complexes by Blue Native Gel Electrophoresis

Published on: September 28, 2018

Denaturing Gradient Gel Electrophoresis (DGGE)

Denaturing Gradient Gel Electrophoresis (DGGE)

Published on: February 25, 2007

Native gel electrophoresis allows analysis of protein size and subunit composition.
Protein mobility is influenced by size, shape, and intrinsic charge.
Continuous PAGE offers flexibility across a wide pH range.

Conclusions:

Nondenaturing gel electrophoresis is a valuable technique for protein analysis.
The choice between continuous and discontinuous PAGE depends on protein characteristics and separation goals.