Related Experiment Video
Updated: Jul 7, 2026

08:33
Labelling and Visualization of Mitochondrial Genome Expression Products in Baker's Yeast Saccharomyces cerevisiae
Published on: April 11, 2021
Metabolic labeling and immunoprecipitation of yeast proteins
1Vanderbilt University, Nashville, Tennessee, USA.
Current Protocols in Cell Biology
|January 30, 2008
Abstract:
The proteins of Saccharomyces cervsiae can be metabolically labeled, as described here, with (35)methionine and (35)cysteine or a hydrolysate of E. coli labeled with (35)O4(2-). After the labeling, protocols are provided for the mechanical disruption of the yeast cells or conversion to spheroplasts, with subsequent lysis before immunoprecipitation of the proteins.

