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Visualizing Low-Abundance Proteins and Post-Translational Modifications in Living Drosophila Embryos via Fluorescent Antibody Injection
Published on: January 19, 2024
Metabolic labeling and immunoprecipitation of Drosophila proteins
1Georgia Institute of Technology, Atlanta, Georgia.
Current Protocols in Cell Biology
|January 30, 2008
Abstract:
The genetics of Drosophila is a powerful tool in the analysis of mutants and mutant proteins. Cultures of cells derived from wild-type or mutant flies can be pulse labeled to biosynthetically label the proteins made by the cells. Immunoprecipitation (and subcellular fractionation) are used to characterize the expression of specific proteins.

