Partial purification of alpha-hemolysin in Escherichia coli
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F Nistiar1, V Janigová, J Durovicová
1Department of Microbiology, Faculty of Medicine, P.J. Safárik University, Kosice, Czechoslovakia.
Folia Microbiologica
|January 1, 1991
Abstract:
The present paper describes isolation and purification of alpha-hemolysin of Escherichia coli. The optimum production medium was found to be the Todd-Hewitt broth. Out of thirteen fractions obtained after separation on Sephadex G-200, two fractions possessed the highest relative specific activity.