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Related Experiment Videos

Analysis of mitochondrial dysfunction during cell death.

Vladimir Gogvadze1, Sten Orrenius, Boris Zhivotovsky

  • 1Institute of Environmental Medicine, Karolinska Institutet, Stockholm, Sweden.

Current Protocols in Cell Biology
|January 30, 2008
PubMed
Summary
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This study details protocols for assessing mitochondrial function during apoptosis. These methods help understand cell death mechanisms involving mitochondria and released proteins.

Area of Science:

  • Cell Biology
  • Biochemistry
  • Molecular Biology

Background:

  • Apoptosis, a form of programmed cell death, is crucial for development and disease.
  • Mitochondria play a central role in initiating and executing apoptosis.
  • Identifying common steps in apoptosis across different stimuli is essential for understanding cell death.

Purpose of the Study:

  • To provide a comprehensive set of protocols for evaluating mitochondrial involvement in apoptosis.
  • To facilitate the analysis of mitochondrial function during cytotoxic stress.
  • To enable researchers to investigate the role of mitochondria in various apoptotic pathways.

Main Methods:

  • Collection and analysis of proteins released from mitochondria during apoptosis.
  • Detection and characterization of the mitochondrial permeability transition.

Related Experiment Videos

  • Measurement of mitochondrial membrane potential.
  • Isolation and preparation of mitochondria from diverse tissue types.

    • Main Results:

    • Established protocols for assessing key mitochondrial events in apoptosis.
    • Enabled the quantification of mitochondrial dysfunction markers.
    • Provided methods for comparative analysis of mitochondrial roles in cell death.

    Conclusions:

    • Mitochondrial function assessment is critical for understanding apoptotic pathways.
    • The provided protocols offer a robust toolkit for apoptosis research.
    • Further investigation into mitochondrial roles can reveal therapeutic targets for diseases involving aberrant cell death.