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Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Updated: Jul 7, 2026

Flow Cytometric Analysis of Apoptotic Biomarkers in Actinomycin D-Treated SiHa Cervical Cancer Cells
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Flow Cytometric Analysis of Apoptotic Biomarkers in Actinomycin D-Treated SiHa Cervical Cancer Cells

Published on: August 26, 2021

Flow cytometry of apoptosis.

Piotr Pozarowski1, Jerzy Grabarek, Zbigniew Darzynkiewicz

  • 1School of Medicine, Lublin, Poland.

Current Protocols in Cell Biology
|January 30, 2008
PubMed
Summary

Flow cytometry is a powerful tool for identifying and quantifying cell death, distinguishing between apoptosis and necrosis. This method allows for the detailed study of cell death mechanisms using multiparametric measurements.

Area of Science:

  • Cell Biology
  • Immunology

Background:

  • Cell death is a fundamental biological process.
  • Distinguishing between apoptosis and necrosis is crucial for understanding cellular health and disease.
  • Flow cytometry offers advanced capabilities for cell analysis.

Purpose of the Study:

  • To outline the utility of flow cytometry in studying cell death.
  • To detail methods for identifying and quantifying cell death.
  • To explore the mechanisms of apoptosis and necrosis.

Main Methods:

  • Flow cytometry utilizing laser light scattering for morphological changes.
  • Fluorochrome reporters and antibodies for detecting early apoptotic events like mitochondrial potential dissipation and caspase activation.
  • Annexin V binding for externalized phosphatidylserine detection.

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Last Updated: Jul 7, 2026

Flow Cytometric Analysis of Apoptotic Biomarkers in Actinomycin D-Treated SiHa Cervical Cancer Cells
13:53

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Published on: August 26, 2021

Detection and Isolation of Apoptotic Bodies to High Purity
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Detection and Isolation of Apoptotic Bodies to High Purity

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  • DNA fragmentation assays (TUNEL, ISEL) for apoptotic DNA damage.
  • Tissue transglutaminase (TGase) activity assessment.

    • Main Results:

    • Flow cytometry can accurately identify and quantify dead or dying cells.
    • The method allows for differentiation between apoptotic and necrotic cell death pathways.
    • Key molecular events in apoptosis, including chromatin condensation, mitochondrial changes, caspase activation, phosphatidylserine exposure, DNA fragmentation, and TGase activation, are detectable.
    • Multiparametric analysis provides a comprehensive view of cell attributes.

    Conclusions:

    • Flow cytometry is a versatile and powerful technique for the comprehensive analysis of cell death.
    • It enables detailed investigation into the mechanisms of apoptosis and necrosis.
    • The ability to perform multiparametric measurements enhances the understanding of cellular responses to death stimuli.