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Establishing a High Throughput Epidermal Spheroid Culture System to Model Keratinocyte Stem Cell Plasticity
Published on: January 30, 2021
Preparation of human epidermal keratinocyte cultures
1National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland, USA.
Current Protocols in Cell Biology
|January 30, 2008
Abstract:
Cultured keratinocytes have been used by a number of investigators in studies investigating wound repair and carcinogenesis, and they have also proven useful as a model for differentiation. This unit describes a protocol for establishing human keratinocytes in tissue culture. Human newborn foreskins are proteolytically digested to separate the epidermis and the dermis, and keratinocytes are obtained from the epidermis.
