Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Comparative genomic hybridization.

Jane Bayani1, Jeremy A Squire

  • 1Princess Margaret Hospital and The Ontario Cancer Institute University of Toronto, Toronto, Ontario, Canada.

Current Protocols in Cell Biology
|January 30, 2008
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Targeting B7-H3 in patients with metastatic triple-negative breast cancer and brain metastases: an opportunity to "revive" immunotherapy as a treatment strategy?

Journal for immunotherapy of cancer·2026
Same author

Population-Level Resources and Costs Associated with Low-Risk Prostate Cancer Patients on Active Surveillance.

Cancers·2026
Same author

Impact of Genomic Mutations on the Transcriptional Pathways and Tumor Microenvironment Landscape of Localized Early Prostate Cancer.

The Prostate·2026
Same author

Transfer Learning and Machine Learning for Training Five-Year Survival Prognostic Models in Early Breast Cancer: Development and Validation Study.

Journal of medical Internet research·2026
Same author

Heterogeneity and immune microenvironment of early invasive estrogen receptor-positive breast cancer reveal an immune-rich subset.

NPJ breast cancer·2026
Same author

Prognostic and predictive performance of PREDICT 2.1, PREDICT v3, and RSClin in node-negative early breast cancer: a TEAM pathology substudy.

Breast cancer research and treatment·2026
Same journal

Measuring Mitochondrial Respiration in Previously Frozen Biological Samples.

Current protocols in cell biology·2020
Same journal

Proximity Ligation Assay for Detecting Protein-Protein Interactions and Protein Modifications in Cells and Tissues in Situ.

Current protocols in cell biology·2020
Same journal

Methods for Investigating Corneal Cell Interactions and Extracellular Vesicles In Vitro.

Current protocols in cell biology·2020
Same journal

Multiplexed Proximity Biotinylation Coupled to Mass Spectrometry for Defining Integrin Adhesion Complexes.

Current protocols in cell biology·2020
Same journal

Preparation of Extracellular Matrix Paper and Construction of Multi-Layered 3D Tissue Model.

Current protocols in cell biology·2020
Same journal

Metabolic Analysis at the Nanoscale with Multi-Isotope Imaging Mass Spectrometry (MIMS).

Current protocols in cell biology·2020
See all related articles

Comparative genomic hybridization (CGH) uses fluorescence in situ hybridization (FISH) to detect DNA copy number changes. This method compares tumor DNA to normal DNA, highlighting genomic alterations for cancer research.

Area of Science:

  • Genomics
  • Molecular Biology
  • Cytogenetics

Background:

  • Comparative genomic hybridization (CGH) is a molecular cytogenetic technique.
  • It is based on fluorescence in situ hybridization (FISH) for detecting chromosomal copy number variations.

Purpose of the Study:

  • To describe the principles and methodology of CGH.
  • To outline the basic FISH techniques applicable to CGH.

Main Methods:

  • CGH involves hybridizing differentially labeled test (tumor) and reference (normal) DNA to a normal metaphase spread.
  • Fluorochromes (e.g., green for test, red for reference) are used for detection.
  • Image analysis software quantifies the ratio of fluorescence signals along each chromosome to identify copy number gains or losses.

Related Experiment Videos

Main Results:

  • Differences in green/red fluorescence ratios indicate relative copy number changes in the test DNA.
  • CGH allows for the screening of the entire genome for amplifications and deletions.

Conclusions:

  • CGH is a powerful screening tool for detecting genomic imbalances.
  • Understanding basic FISH techniques is crucial for successful CGH analysis.