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Related Experiment Video

Updated: Jul 7, 2026

Reconstitution of Cell-cycle Oscillations in Microemulsions of Cell-free Xenopus Egg Extracts

Reconstitution of Cell-cycle Oscillations in Microemulsions of Cell-free Xenopus Egg Extracts

Published on: September 27, 2018

Analysis of the cell cycle using Xenopus egg extracts.

Sally Kornbluth¹, Jing Yang, Maureen Powers

¹Duke University Medical Center, Durham, North Carolina, USA.

Current Protocols in Cell Biology

|January 30, 2008

Summary

Researchers developed a Xenopus egg extract system that supports nuclear assembly and DNA replication. This cell-free system is crucial for studying fundamental processes like DNA replication and nuclear transport in a controlled environment.

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Area of Science:

Cell Biology
Molecular Biology
Developmental Biology

Background:

Cell cycle regulation is fundamental to eukaryotic life.
Understanding DNA replication and nuclear transport requires accessible experimental systems.

Purpose of the Study:

To establish a cell-free system for studying DNA replication.
To enable research on nuclear transport mechanisms.
To utilize Xenopus egg extracts for cell cycle studies.

Main Methods:

Isolation of Xenopus eggs from hormonally primed female frogs.
Treatment of egg extract with cycloheximide to maintain interphase.
Addition of sperm chromatin and ATP to induce nuclear assembly.

Main Results:

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A Cell Free Assay to Study Chromatin Decondensation at the End of Mitosis

Published on: December 19, 2015

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Last Updated: Jul 7, 2026

Reconstitution of Cell-cycle Oscillations in Microemulsions of Cell-free Xenopus Egg Extracts

Reconstitution of Cell-cycle Oscillations in Microemulsions of Cell-free Xenopus Egg Extracts

Published on: September 27, 2018

Xenopus laevis Egg Extract Preparation and Live Imaging Methods for Visualizing Dynamic Cytoplasmic Organization

Xenopus laevis Egg Extract Preparation and Live Imaging Methods for Visualizing Dynamic Cytoplasmic Organization

Published on: June 6, 2021

A Cell Free Assay to Study Chromatin Decondensation at the End of Mitosis

A Cell Free Assay to Study Chromatin Decondensation at the End of Mitosis

Published on: December 19, 2015

Successful isolation and preparation of Xenopus egg extract.
Demonstration of nuclear envelope formation and chromatin decondensation.
Creation of a functional system for studying DNA replication and nuclear transport.

Conclusions:

Xenopus egg extract provides a robust platform for investigating nuclear assembly.
The established system facilitates research into DNA replication dynamics.
This method is valuable for dissecting nuclear transport pathways.