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Updated: Jul 7, 2026
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Reconstitution of Cell-cycle Oscillations in Microemulsions of Cell-free Xenopus Egg Extracts
Published on: September 27, 2018
Analysis of the cell cycle using Xenopus egg extracts
Sally Kornbluth1, Jing Yang, Maureen Powers
1Duke University Medical Center, Durham, North Carolina, USA.
Current Protocols in Cell Biology
|January 30, 2008
Abstract:
In this unit, Xenopus eggs are isolated from hormonally primed female frogs, and then the extract is treated with cyclohexamide so it remains in interphase of the cell cycle. In the presence of sperm chromatin and ATP, membrane vesicles in the extract fuse to assemble nuclei, making the extract suitable for studies of DNA replication and nuclear transport.
