Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Tetraspanin CD82 shapes EGFR signaling outcomes through nanoscale receptor organization.

The Journal of cell biology·2026

Same author

IgE occupancy and antigen valency cooperate to control FcεRI aggregation geometry and signaling efficiency.

bioRxiv : the preprint server for biology·2025

Same author

Mathematical models for the EP2 and EP4 signaling pathways and their crosstalk.

Journal of theoretical biology·2025

Same author

Building a dynamic model for EGFR-family dimerization using quantitative, live-cell fluorescence methods.

Biophysical journal·2025

Same author

Patient-derived ovarian cancer models demonstrate the influence of tumor-associated macrophages on therapeutic response.

Oncoimmunology·2025

Same author

Fast Single-Particle Tracking of Membrane Proteins Combined with Super-Resolution Imaging of Actin Nanodomains.

Scientific data·2025

Search research articles

Related Experiment Video

Updated: Jul 7, 2026

In Vivo Optical Imaging of Brain Tumors and Arthritis Using Fluorescent SapC-DOPS Nanovesicles

In Vivo Optical Imaging of Brain Tumors and Arthritis Using Fluorescent SapC-DOPS Nanovesicles

Published on: May 2, 2014

In vivo imaging using quantum-dot-conjugated probes.

Diane S Lidke¹, Peter Nagy, Donna J Arndt-Jovin

¹University of New Mexico, Albuquerque, New Mexico, USA.

Current Protocols in Cell Biology

|January 30, 2008

Summary

Quantum dots (QDs) offer superior brightness and photostability for live-cell imaging and quantitative flow cytometry. Their unique properties overcome limitations of conventional fluorophores, enabling precise analysis of cellular processes and receptor interactions.

More Related Videos

Production and Targeting of Monovalent Quantum Dots

Production and Targeting of Monovalent Quantum Dots

Published on: October 23, 2014

Visualizing Subcellular Localization of a Protein in the Heart Using Quantum Dots-Mediated Immuno-Labeling Followed by Transmission Electron Microscopy

Visualizing Subcellular Localization of a Protein in the Heart Using Quantum Dots-Mediated Immuno-Labeling Followed by Transmission Electron Microscopy

Published on: September 16, 2022

Related Experiment Videos

Last Updated: Jul 7, 2026

In Vivo Optical Imaging of Brain Tumors and Arthritis Using Fluorescent SapC-DOPS Nanovesicles

In Vivo Optical Imaging of Brain Tumors and Arthritis Using Fluorescent SapC-DOPS Nanovesicles

Published on: May 2, 2014

Production and Targeting of Monovalent Quantum Dots

Production and Targeting of Monovalent Quantum Dots

Published on: October 23, 2014

Visualizing Subcellular Localization of a Protein in the Heart Using Quantum Dots-Mediated Immuno-Labeling Followed by Transmission Electron Microscopy

Visualizing Subcellular Localization of a Protein in the Heart Using Quantum Dots-Mediated Immuno-Labeling Followed by Transmission Electron Microscopy

Published on: September 16, 2022

Area of Science:

Biotechnology
Cell Biology
Nanotechnology

Background:

Conventional fluorophores and visible fluorescent proteins (VFPs) have limitations including small Stokes' shift and photobleaching.
These limitations hinder prolonged imaging and quantitative analysis in live-cell studies.

Purpose of the Study:

To describe the application of quantum dots (QDs) for advanced live-cell imaging.
To detail the use of QDs in flow cytometry for quantitative analysis of ligand binding and receptor density.

Main Methods:

Utilizing streptavidin-coupled QDs for broad applicability to cell surface receptors.
Coupling ligands or antibodies to biotin for visualization with QDs.

Main Results:

QDs demonstrate high brightness and exceptional photostability compared to conventional fluorophores.
This enables detailed visualization and quantitative analysis in live-cell environments.

Conclusions:

QDs represent an advanced tool for live-cell imaging and quantitative flow cytometry.
Their photostability and brightness overcome limitations of traditional fluorescent probes.