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Related Concept Videos

Genetic Screens02:46

Genetic Screens

Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing  genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which result in visible changes...

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Investigating Protein-protein Interactions in Live Cells Using Bioluminescence Resonance Energy Transfer
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Investigating Protein-protein Interactions in Live Cells Using Bioluminescence Resonance Energy Transfer

Published on: May 26, 2014

The BRET technology and its application to screening assays.

Johan Bacart1, Caroline Corbel, Ralf Jockers

  • 1UMR 8090, CNRS, Université Lille 2, Lille, France.

Biotechnology Journal
|January 30, 2008
PubMed
Summary

Bioluminescence resonance energy transfer (BRET) is a versatile method for studying protein interactions in living cells. BRET technology is crucial for identifying new therapeutic compounds, particularly inhibitors of protein-protein interactions, aiding drug screening efforts.

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Biotechnology

Background:

  • Bioluminescence resonance energy transfer (BRET) is a biophysical technique developed in 1999.
  • It relies on energy transfer between a light-emitting enzyme (donor) and a fluorescent molecule (acceptor).
  • Various BRET methodologies and donor/acceptor pairs have been developed since its inception.

Purpose of the Study:

  • To review the original BRET methodology and its recent advancements.
  • To highlight the utility of BRET in studying protein-protein interactions in living cells.
  • To discuss the application of BRET in drug screening, particularly for identifying novel therapeutic agents.

Main Methods:

  • Description of the fundamental principles of BRET.
  • Overview of different BRET variants and their specific applications.
  • Discussion of BRET-based assays used in drug discovery.

Main Results:

  • BRET is a highly versatile technique for real-time analysis of protein-protein interactions.
  • BRET assays have been successfully employed in screening for receptor ligands and protease inhibitors.
  • The technology shows significant promise for identifying inhibitors of protein-protein interactions.

Conclusions:

  • BRET technology is a powerful tool for understanding dynamic biological processes.
  • Its application in drug screening is expanding, especially for targeting protein-protein interactions.
  • BRET is expected to be instrumental in the development of future therapeutics.