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Related Experiment Videos

Cestode vaccines.

M D Rickard1

  • 1CSIRO Division of Animal Health, Parkville, Victoria, Australia.

The Southeast Asian Journal of Tropical Medicine and Public Health
|December 1, 1991
PubMed
Summary
This summary is machine-generated.

Developing effective vaccines against larval cestode infections is possible using recombinant DNA technology. A stabilized fusion protein antigen from Taenia ovis demonstrated high protection in sheep, overcoming previous instability issues.

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Area of Science:

  • Veterinary Immunology
  • Parasitology
  • Biotechnology

Background:

  • Larval cestode infections pose significant threats to human and animal health.
  • Domesticated animals serve as intermediate hosts for key cestode infections like hydatidosis and cysticercosis.
  • Natural immunity in intermediate hosts can be induced via vaccination with oncosphere antigens, but antigen supply has been a challenge.

Purpose of the Study:

  • To explore the feasibility of developing commercial cestode vaccines using recombinant DNA technology.
  • To stabilize a previously identified vaccine antigen for improved commercial production.
  • To assess the immunogenicity and protective efficacy of modified vaccine antigens.

Main Methods:

  • Construction of a cDNA library from Taenia ovis oncospheres.

Related Experiment Videos

  • Expression of T. ovis polypeptide antigen 45W as a fusion protein with Schistosoma japonicum glutathione S-transferase (GST-45W).
  • Stabilization of the fusion protein by deleting carboxyl-terminal residues and enzymatic cleavage of the antigen.

    • Main Results:

    • The initial GST-45W fusion protein provided up to 94% protection in sheep against challenge infection.
    • The vaccine antigen was found to be unstable, hindering commercial production efforts.
    • A stabilized fusion protein (GST-45W (B/X)) and the cleaved 45W polypeptide retained high host-protective efficacy.

    Conclusions:

    • Recombinant DNA technology offers a viable approach for producing effective cestode vaccine antigens.
    • Stabilization of the antigen is crucial for overcoming production challenges and ensuring commercial viability.
    • The T. ovis 45W polypeptide is immunogenic and protective, independent of the GST fusion partner.