Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Functional PEG-modified thin films for biological detection.

Aaron S Anderson1, Andrew M Dattelbaum, Gabriel A Montaño

  • 1Los Alamos National Laboratory, Los Alamos, New Mexico 87545, USA.

Langmuir : the ACS Journal of Surfaces and Colloids
|January 31, 2008
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

An Elastin-like Polymer Targeting Vascular Endothelial Growth Factor Receptor-1 Reduces Survival in Serum-Starved Endothelial Cells.

Biochemical engineering journal·2025
Same author

Atomically Precise Nanoclusters as Co-Catalysts for Light-Activated Microswimmer Motility.

Small (Weinheim an der Bergstrasse, Germany)·2025
Same author

Reactivity of [(PNP)Mn(CO)<sub>2</sub>] with Organophosphates.

ACS organic & inorganic Au·2023
Same author

Potent and selective covalent inhibition of the papain-like protease from SARS-CoV-2.

Nature communications·2023
Same author

Label-free affinity screening, design and synthesis of inhibitors targeting the Mycobacterium tuberculosis L-alanine dehydrogenase.

PloS one·2022
Same author

PCP consensus protein/peptide alphavirus antigens stimulate broad spectrum neutralizing antibodies.

Peptides·2022
Same journal

Synergistic Visible-Light-Driven CO<sub>2</sub> Reduction and H<sub>2</sub>O Oxidation over Ti<sub>3</sub>C<sub>2</sub> Quantum Dot-Modified Cu/g-C<sub>3</sub>N<sub>4</sub> Photocatalysts.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Spontaneous Phase Separation Enables Rapid, Polymerization-Free Fabrication of Gels.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Lamellar-Confinement-Induced ZIF-67 Nanosheet Mixed Matrix Membranes for Enhanced CH<sub>4</sub>/N<sub>2</sub> Separation.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Structure Control of Oblate Nanoparticles Self-Assembled by ABC Cyclic Terpolymers under Soft Confinement.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Tuning Brønsted/Lewis Acid Site Ratios via Ammonia Modulation for Selective Conversion of Glycerol to 1,3-Propanediol or Solketal.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Catalytic and Nitriding Competition of Nitrogen Atom on Graphene and Its Finite Rate Surface Chemistry Model.

Langmuir : the ACS journal of surfaces and colloids·2026
See all related articles

Researchers developed a method to create functional organic thin films on silica surfaces for biological assays. These films improve biosensor specificity and reduce nonspecific binding, paving the way for robust biosensor development.

Area of Science:

  • Materials Science
  • Biotechnology
  • Surface Chemistry

Background:

  • Developing functional organic thin films is crucial for sensitive and specific biological assays.
  • Oxide surfaces require effective functionalization for biomolecule immobilization and assay performance.
  • Existing methods often face challenges with uniformity and nonspecific binding.

Purpose of the Study:

  • To establish a general procedure for preparing functional organic thin films on oxide surfaces.
  • To optimize the deposition of amine-terminated silane films for high-quality monolayer formation.
  • To create surfaces with specific biomolecular recognition capabilities for biosensing applications.

Main Methods:

  • Functionalization of silica surfaces using vapor-phase deposition of 3'-aminopropylmethyldiethoxysilane (APMDES).

Related Experiment Videos

  • Characterization of film quality using atomic force microscopy (AFM), ellipsometry, and contact angle measurements.
  • Chemical modification of amine-terminated films with biotinylated poly(ethylene glycol) (PEG) chains.

    • Main Results:

    • Vapor-phase deposition of APMDES yielded uniform, high-quality monolayer films.
    • Biotinylated PEG surfaces demonstrated specific affinity for streptavidin.
    • Functional PEG surfaces significantly reduced nonspecific binding in pseudo-sandwich assays for Bacillus anthracis protective antigen (PA) detection.
    • Successful PA detection was achieved in both buffer and complex media.

    Conclusions:

    • The developed procedure provides a robust method for creating functional organic thin films on oxide surfaces.
    • These functionalized films enhance specificity and reduce background noise in biosensing applications.
    • The findings represent a significant advancement towards the development of autonomous and reusable biosensors.