[In vitro toxicity evaluation of 12 kinds of dental materials using a modified cell culture technique]

In view of the fact that up to date the various cell toxicity tests could not come to an identical result and compare with each other. This paper, referring to the covering documents of F.D.I. and I.S.O., summing up the principles and characteristics of various cell culture techniques, establishes a modified cell toxicity test. This method, with more respects of wide observations and tangible results, is easy to grasp, well to repeat and can be reserved for checking the results, meanwhile, it can be avoided the contradiction caused by the different experiments on one kind of material. Therefore, it is very worthy of appraising the biological safety of the materials. The study of 12 kinds of dental materials expressed that the toxicity order of copper alloy was mean value of 0 (Jiao Tong University), copper alloy tridium was mean value of 3 (U.S.A.), copper alloy T was mean value of 2 (Jiao Tong University) and the others were mean value of 0-1. This paper also discussed several problems with common concerns in studying the cell toxicity of the biomaterials.