Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Production of Organic Acids

Production of Organic Acids

Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...

Production of Antibiotics

Production of Antibiotics

Penicillin, one of the earliest and most widely used antibiotics, is produced industrially by the filamentous fungus Penicillium chrysogenum. Large stirred-tank bioreactors ranging from tens to hundreds of thousands of liters maintain tightly controlled temperature, pH, and dissolved oxygen conditions to support fungal metabolism and maximize antibiotic yield. Penicillin is a secondary metabolite, synthesized primarily during the stationary growth phase, which requires a carefully managed...

Biofuels

Biofuels

The microbial conversion of organic matter into biofuels holds potential as a renewable energy source. Among biofuel sources, microalgae are recognized as a highly efficient and adaptable feedstock for biodiesel production, owing to their rapid biomass accumulation, elevated lipid productivity, and capacity to proliferate in diverse aquatic systems, including freshwater, marine, and wastewater habitats. Unlike terrestrial crops, microalgae do not compete for land and can achieve significantly...

Production of Pharmaceuticals

Production of Pharmaceuticals

Industrial insulin production uses genetically engineered E. coli expressing a proinsulin gene controlled by a tryptophan promoter and containing a methionine linker for later cleavage. The cells also carry ampicillin resistance for selective growth. Seed cultures are stored at −80 °C and production begins by thawing a small amount to inoculate starter cultures, which are progressively scaled to a 50,000-L bioreactor. In the bioreactor, E. coli grow in nutrient-rich media under sterile, tightly...

Bioreactor Design and Operational System

Bioreactor Design and Operational System

Bioreactors are engineered vessels designed to cultivate microorganisms under controlled conditions for industrial bioprocessing. They maintain sterility and allow precise regulation of pH, temperature, oxygen, and nutrient levels to optimize microbial growth and metabolite production. Bioreactors range from small laboratory units of 1 liter to industrial systems holding up to 500,000 liters, though only about 75% of their volume is actively used for fermentation. The remaining headspace...

Upstream Processing

Upstream Processing

Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Association of Bioactive Compounds With Antioxidant and Antimicrobial Activities of Allium Extracts Prepared With Commercial Rice Wines and Kaoliang Liquors.

Journal of nutrition and metabolism·2026

Same author

Antioxidant and antimicrobial activities of commercial rice wine extracts of Taiwanese Allium fistulosum.

Food chemistry·2015

Same author

Effects of vegetation type on microbial biomass carbon and nitrogen in subalpine mountain forest soils.

Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi·2014

Same author

Protein enrichment and digestion improvement of napiergrass and pangolagrass with solid-state fermentation.

Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi·2012

Same author

Protein enrichment, cellulase production and in vitro digestion improvement of pangolagrass with solid state fermentation.

Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi·2011

Same author

Microbial communities and bacterial diversity of spruce, hemlock and grassland soils of Tatachia Forest, Taiwan.

Journal of environmental science and health. Part. B, Pesticides, food contaminants, and agricultural wastes·2010

Search research articles

Related Experiment Video

Updated: Jul 7, 2026

Continuous Liquid-Liquid Extraction of Medium-Chain Fatty Acids from Fermentation Broth Using Hollow-Fiber Membranes

Continuous Liquid-Liquid Extraction of Medium-Chain Fatty Acids from Fermentation Broth Using Hollow-Fiber Membranes

Published on: August 9, 2024

Polyunsaturated fatty acids production with a solid-state column reactor.

Hung-Der Jang¹, Shang-Shyng Yang

¹Department of Food Science, Yuanpei University, Hsinchu 300, Taiwan.

Bioresource Technology

|January 31, 2008

Summary

This study optimized polyunsaturated fatty acid (PUFA) production using rice bran and Mortierella alpina. Optimal conditions yielded significant amounts of total PUFAs, including eicosapentaenoic acid (EPA) and arachidonic acid (AA).

More Related Videos

Experimental Protocol for Biodiesel Production with Isolation of Alkenones as Coproducts from Commercial Isochrysis Algal Biomass

Experimental Protocol for Biodiesel Production with Isolation of Alkenones as Coproducts from Commercial Isochrysis Algal Biomass

Published on: June 24, 2016

Laboratory Production of Biofuels and Biochemicals from a Rapeseed Oil through Catalytic Cracking Conversion

Laboratory Production of Biofuels and Biochemicals from a Rapeseed Oil through Catalytic Cracking Conversion

Published on: September 2, 2016

Related Experiment Videos

Last Updated: Jul 7, 2026

Continuous Liquid-Liquid Extraction of Medium-Chain Fatty Acids from Fermentation Broth Using Hollow-Fiber Membranes

Continuous Liquid-Liquid Extraction of Medium-Chain Fatty Acids from Fermentation Broth Using Hollow-Fiber Membranes

Published on: August 9, 2024

Experimental Protocol for Biodiesel Production with Isolation of Alkenones as Coproducts from Commercial Isochrysis Algal Biomass

Experimental Protocol for Biodiesel Production with Isolation of Alkenones as Coproducts from Commercial Isochrysis Algal Biomass

Published on: June 24, 2016

Laboratory Production of Biofuels and Biochemicals from a Rapeseed Oil through Catalytic Cracking Conversion

Laboratory Production of Biofuels and Biochemicals from a Rapeseed Oil through Catalytic Cracking Conversion

Published on: September 2, 2016

Area of Science:

Biotechnology
Food Science
Microbial Fermentation

Background:

Polyunsaturated fatty acids (PUFAs) are essential nutrients with significant health benefits.
Microbial fermentation offers a sustainable route for PUFA production.
Rice bran is an abundant agricultural byproduct with potential as a fermentation substrate.

Purpose of the Study:

To investigate the potential for producing PUFAs using a solid-state column reactor with rice bran and Mortierella alpina.
To determine the optimal conditions for maximizing PUFA yield and specific fatty acid profiles.

Main Methods:

Utilized a solid-state column reactor system.
Optimized fermentation parameters including nitrogen source supplementation, initial moisture content, pH, aeration, and temperature profiles.
Incubation involved a two-stage temperature strategy (20°C for 5 days, then 12°C for 7 days).

Main Results:

Optimal conditions achieved 127 mg total PUFAs per gram of substrate carbon.
Specific yields included 12 mg eicosapentaenoic acid (EPA), 6 mg arachidonic acid (AA), 5 mg alpha-linolenic acid (ALA), and 117 mg linoleic acid (LA).
Aeration significantly enhanced the production of AA, EPA, and total PUFAs. Nitrogen supplementation and temperature shifts further boosted EPA production.

Conclusions:

Solid-state fermentation of rice bran by Mortierella alpina is an effective method for PUFA production.
Optimized conditions, including specific aeration and temperature control, maximize yield and desirable fatty acid profiles.
This approach presents a viable strategy for sustainable production of valuable fatty acids like EPA and AA.