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Hybridoma Technology

Hybridoma Technology

Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation, polyethylene glycol...

Antibody Structure

Antibody Structure

Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
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Immunoprecipitation

Immunoprecipitation

Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
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Related Experiment Video

Updated: Jul 7, 2026

Generation of Murine Monoclonal Antibodies by Hybridoma Technology

Generation of Murine Monoclonal Antibodies by Hybridoma Technology

Published on: January 2, 2017

Production of polyclonal antisera.

Helen M Cooper¹, Yvonne Paterson

¹Queensland Brain Institute and University of Queensland, Brisbane, Australia.

Current Protocols in Molecular Biology

|January 31, 2008

Summary

This summary is machine-generated.

Generating specific polyclonal antibodies is crucial for biological research techniques. This guide details methods for producing these essential antibodies in various animals like rabbits and mice.

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Last Updated: Jul 7, 2026

Generation of Murine Monoclonal Antibodies by Hybridoma Technology

Generation of Murine Monoclonal Antibodies by Hybridoma Technology

Published on: January 2, 2017

Generation of Monoclonal Antibodies Against Natural Products

Generation of Monoclonal Antibodies Against Natural Products

Published on: April 6, 2019

Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood

Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood

Published on: February 6, 2018

Area of Science:

Biochemistry
Immunology
Molecular Biology

Background:

Specific antibodies are essential for numerous biological and biochemical techniques, including immunohistochemistry, ELISAs, immunoprecipitation, and immunoblotting.
The demand for specific antibodies is increasing due to antibody-based proteomic strategies for protein expression and post-translational modification analysis.

Purpose of the Study:

To provide standardized protocols for the production of polyclonal antisera specific for protein antigens.
To facilitate the generation of large quantities of specific antibodies for basic and applied research.

Main Methods:

Immunization of laboratory animals (rabbits, rats, mice, hamsters) with purified protein or peptide antigens.
Collection and processing of antisera to yield specific polyclonal antibodies.

Main Results:

Established protocols for generating polyclonal antibodies in multiple animal models.
Demonstrated the value of animal-derived polyclonal antibodies for laboratory applications.

Conclusions:

The described protocols enable efficient production of specific polyclonal antibodies, supporting diverse research needs.
Availability of reliable antibody production methods is critical for advancing biological and biochemical research programs.