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Real-Time Detection and Capture of Invasive Cell Subpopulations from Co-Cultures

Real-Time Detection and Capture of Invasive Cell Subpopulations from Co-Cultures

Published on: March 30, 2022

Cell culture imaging using microimpedance tomography.

Pontus Linderholm¹, Laurent Marescot, Meng Heng Loke

¹Laboratory of Microsystems, Ecole Polytechnique Fédérale Lausanne, LMIS, Station 17, 1015 Lausanne, Switzerland. pontus.linderholm@epfl.ch

IEEE Transactions on Bio-Medical Engineering

|February 1, 2008

Summary

This summary is machine-generated.

We developed a low-cost, rapid microimpedance tomography system for 2D cell and tissue imaging. This technology can monitor cell growth and electroporation effects, offering insights into cell membrane changes.

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Area of Science:

Biomedical Engineering
Cell Biology
Imaging Technologies

Background:

Accurate imaging of cell and tissue cultures is crucial for biological research.
Existing methods may be expensive, slow, or lack resolution for specific applications.
Monitoring cellular processes in real-time requires advanced imaging techniques.

Purpose of the Study:

To introduce a novel, inexpensive, and fast microimpedance tomography system.
To demonstrate the system's capability for 2D imaging of cell and tissue cultures.
To explore the system's potential in studying cellular changes like electroporation.

Main Methods:

Utilizing a four-electrode measurement setup with 16 planar microelectrodes integrated into a culture chamber.
Employing an Agilent 4294A impedance analyzer and a front-end amplifier for impedance measurements.
Applying a reconstruction algorithm to generate two-dimensional images from impedance data.

Main Results:

The system accurately resolved the shape and position of a human hair, providing vertical cross sections.
Human epithelial stem cells (YF 29) were successfully cultured directly on the device.
Tissue growth was monitored over several days, and a decrease in resistivity due to cell membrane permeabilization was observed.

Conclusions:

The developed microimpedance tomography system offers a cost-effective and rapid solution for 2D cell and tissue imaging.
The system can track tissue growth and detect changes in cell membrane integrity.
This technique shows promise for applications in electroporation studies and real-time monitoring of cellular processes.