Proposal of an experimental model for the study of polycystic ovaries

In view of the correlation of the circadian system with the estrous cycle in the rat, some investigators have proposed the induction of polycystic ovaries by exposing rats to continuous light. However, there is divergence with respect to time of exposure and light intensity. In the present paper we propose a simple and efficient experimental model for the study of polycystic ovarian physiopathology in rats. Twenty female Wistar rats weighing 200-220 g were initially submitted to a 14-h light/10-h dark cycle. Of the animals with regular estrous cycles, 9 were exposed to continuous light (600 lux) for 74 days (group 1) and 6 remained under the initial lighting conditions. A daily vaginal smear was used as indirect evidence for the changes in the estrous cycle. Plasma gonadotropin measurement and histological ovarian analysis showed that 90% of the animals in group 1 developed cystic follicles with the absence of the corpus luteum, despite the lack of reduction in ovarian weight commonly observed under 24-h lighting. This observation may be explained by the reduced time of stress to which the animals were submitted, since polycystic ovarian development occurred within less than 75 days of continuous exposure to 600-lux light, a time relatively shorter than commonly reported in the literature.