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Differential regulation of insulin-like growth factor binding protein-1 and -2 by insulin in the baboon (Papio
Steven D Fleming1, Asgerally T Fazleabas, Stephen C Bell
1Department of Obstetrics and Gynecology (MC808), College of Medicine, University of Illinois at Chicago, Illinois 60612-9998, USA. stevenf@westgate.wh.usyd.edu.au
Background:
The purpose of this study was to examine the effect of insulin on expression and synthesis of IGFBP-1 and IGFBP-2 in the baboon endometrium in vitro.
Methods:
Baboon endometrial explants collected from cycling, ovariectomized, steroid-treated, simulated-pregnant and pregnant animals were cultured for 48 h in the presence or absence of insulin, with or without estradiol, progesterone and hCG.
Results:
Insulin clearly inhibited IGFBP-1 production and mRNA expression in a time- and dose-dependent manner, whereas IGFBP-2 synthesis was not significantly affected. The inhibitory effects of insulin on IGFBP-1 were more evident in explants of non-pregnant tissue or tissue away from the implantation site. In the absence of insulin, synthesis of IGFBP-1 was induced in explants with low levels of de novo synthesis whereas IGFBP-2 synthesis was inhibited. This effect was potentiated by steroids and hCG in the explant cultures.
Conclusion:
Insulin differentially regulates endometrial IGFBP-1 and IGFBP-2 secretion in the baboon.
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