Related Experiment Video
Updated: Jul 7, 2026
In Vitro Culture of Epithelial Cells from Different Anatomical Regions of the Human Amniotic Membrane
Published on: November 28, 2019
Proteomic analysis of human amniotic fluid from Rh(-) pregnancy
Md Imtaiyaz Hassan1, Vijay Kumar, Tej P Singh
1Department of Biophysics, All India Institute of Medical Sciences, New Delhi 110029, India.
Background:
The objective of this study was to identify proteins from the amniotic fluid of the most common reproductive ailment, that is, Rh(-) incompatibility. In the present study, AF from Rh(-) pregnancy in the 29-35th week of gestation was analyzed by 2-DE, and our purpose was to identify the major spots on 2D gel.
Methods:
Proteins from an amniotic fluid sample was separated on 2D gel and visualized after silver staining. Most of the spots were analyzed by MALDI-TOF/MS, and were identified based on the NCBI database by a peptide mass fingerprinting method.
Results:
Albumin, serotransferrin, haptoglobin, alpha-fetoprotein and immunoglobulin were the major proteins detected. On comparison with 2D gel of normal AF, differences were observed at the level of albumin, and newly identified Calgranulins and Neutophil-defensins were observed. Many enzymes, growth factor binding proteins, protease inhibitors and proteins related to inflammation have also been identified.
Conclusions:
This study demonstrates, for the first time, the potential utility of proteomics-based approaches to identify specific biomarkers like Calgranulins and Neutophil-defensins and diagnostic profiles for Rh(-) incompatibility processes and pathophysiologic conditions of pregnancy.
