Determination of nitrate by conversion to nitrite using Paracoccus denitrificans
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I Matchová1, I Cerná, I Kucera
1Department of Biochemistry, Faculty of Science, Masaryk University, Brno, Czechoslovakia.
Folia Microbiologica
|January 1, 1991
Abstract:
A new method of determination of nitrate was developed, utilizing the nitrate reductase activity of Paracoccus denitrificans in which a further reduction of nitrate is blocked either by a mutation affecting formation of cytochromes c or by inhibition of the electron flow to nitrite reductase by mucidin. After deproteinization of the sample with zinc acetate the nitrite produced is determined colorimetrically.