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Sterol composition of nystatin-resistant Candida maltosa mutants
N P Mikhailova1, E F Sorokoletova, E N Durasova
1All-Union Institute of Plant Material Hydrolysis, Leningrad, USSR.
Folia Microbiologica
|January 1, 1991
Abstract:
Composition of sterol fractions of nystatin-resistant Candida maltosa strains was determined. Using UV-spectrometry, TLC and GLC-MS it was demonstrated that resistance to nystatin is connected with the composition alterations of yeast cell sterols. Block of different stages of ergosterol biosynthesis was revealed in some mutants, viz. C-24-transmethylation, delta 8----delta 7-isomerization, 14 alpha-demethylation, C-5(6)-dehydrogenation, reduction of C-14(15) and C-24(28) double bonds.