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Long-term affinity modification on poly(dimethylsiloxane) substrate and its application for ELISA analysis.

Wang-Chou Sung1, Chih-Ching Chang, Honest Makamba

  • 1Department of Chemistry, and Department of Environmental and Occupational Health, National Cheng Kung University, Tainan, 701, Taiwan.

Analytical Chemistry
|February 2, 2008
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel surface modification for poly(dimethylsiloxane) (PDMS) biodevices, creating a stable, hydrophilic affinity surface for biomolecular interactions. This advancement enables sensitive detection of proteins like TGF-beta in biological samples.

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Fabrication of polydimethylsiloxane (PDMS)-Based Flexible Surface-Enhanced Raman Scattering (SERS) Substrate for Ultrasensitive Detection

Published on: November 17, 2023

Area of Science:

  • Biomaterials Science
  • Surface Chemistry
  • Biotechnology

Background:

  • Poly(dimethylsiloxane) (PDMS) is favored for biodevices due to biocompatibility and oxygen permeability.
  • PDMS's hydrophobicity hinders stable, reactive affinity surface creation for biomolecular interactions.
  • Existing methods struggle with long-term stability and reactivity on PDMS substrates.

Purpose of the Study:

  • To engineer a stable, hydrophilic affinity surface on PDMS for enhanced biomolecular detection.
  • To develop a poly(dimethylsiloxane) immunodevice (LPID) for sensitive protein quantification.
  • To investigate the long-term stability and reactivity of the modified PDMS surface.

Main Methods:

  • Surface modification using polyelectrolyte multilayers for hydrophilicity and protein layers (BSA, anti-BSA, Protein G) for affinity.
  • Characterization via atomic force microscopy (AFM), ATR-FT-IR, contact angle, and fluorescence measurements.
  • Fabrication of a long-term PDMS immunodevice (LPID) and application in ELISA for TGF-beta detection in mouse serum.

Main Results:

  • A stable, hydrophilic, and reactive surface was achieved, maintaining properties for over 7 days in dry form.
  • The LPID demonstrated sensitive detection of TGF-beta in mouse serum with a linear range of 500 to 15.125 pg/mL.
  • High precision (RSD < 3%) and accurate quantification of TGF-beta were achieved, eliminating the need for blocking reagents.

Conclusions:

  • The novel surface modification overcomes PDMS hydrophobicity, providing a stable and reactive platform for biodevices.
  • The developed LPID shows significant potential for sensitive and specific protein detection in complex biological matrices.
  • This method is promising for fabricating various PDMS-based affinity devices, including protein chips.