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[Analysis of pde6b mRNA sequences of Kunming mice and C57BL/6J mice]
Guo-lin Yan1, You-sheng Wu, Qun Guo
1Department of Clinical Aerospace Medicine Aerospace Medicine, Fourth Military Medical University, Xi'an 710032, China.
Aim:
To clone and sequence the phosphodiesterase 6 beta subunit (pde6b) gene of Kunming mice, and to compare it with counterpart sequences in the GenBank database.
Methods:
The primers were designed covering the CDS region of the pde6b gene, and the corresponding fragments were amplified using RT-PCR. The fragments were cloned into plasmids, amplified in E.coli, and sequenced. Bioinformatics programs and online tools were used to analyze the sequences.
Results:
The CDS of the pde6b gene of Kunming mice was cloned and sequenced. Compared with the inbred mice C57BL/6J, pde6b CDS sequence of Kunming mice was different in some points: a G-->A transition at +706, a C-->T transversion at +1149. The protein sequence was of little difference: only a glycine-->serine at +236.
Conclusion:
The pde6b CDS region of Kunming mice was successfully cloned. Pde6b sequences are of some level difference in different kinds of mice, but they are most conserved in protein level.
