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Updated: Jul 7, 2026
Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood
Published on: February 6, 2018
[Preparation and identification of monoclonal antibodies against human DcR3]
Chun-feng Wu1, Zhong-sen Ma, Xiu-li Wang
1Department of Respiratory Diseases, Jilin University, Changchun 130041, China. feng1218@sohu.com
Aim:
To prepare monoclonal antibodies(mAbs) against human DcR3 and identify their characterization.
Methods:
BALB/c mice were immunized with purified His-DcR3 protein, and mAbs against DcR3 which prepared by hybridoma technique were purified and identified by their specificity, subtype, titers via ELISA and Western blot.
Results:
Five hybridoma cell lines secreting mAbs against human DcR3 were obtained, which were determined as IgG1 subtype and ascites titers of five mAbs against DcR3 reached 1x10(-5)-1x10(-7). Five mAbs were proved to recognize His-DcR3 protein specifically, one of which (1B1) could recognize SW480 cell.
Conclusion:
mAbs against DcR3 with high titers and specificity have been prepared and purified successfully, which laid a foundation for the study of DcR3 expression, distribution in tissu and development of ELISA kit.
