Related Experiment Video
Updated: Jul 7, 2026

Using the E1A Minigene Tool to Study mRNA Splicing Changes
Published on: April 22, 2021
[Effects of a novel gene NOR(1) on the protein expression of HepG2 cell line]
Xin-min Nie1, Jian-da Zhou, Zhu-ying Huang
1Clinical Laboratory, Central South University, Changsha 410013, China. niexinmin7440@hotmail.com
Aim:
To investigate the mechanism of NOR(1) gene, a novel gene associated with liver cancer.
Methods:
NOR(1) was introduced into HepG2 cells by liposome transfection. After staining and image analysis, 6 differential expression spots which were up-regulated in NOR(1) transfected cells were isolated and identified by two-dimensional polyacrylamide gel eletrophoresis (2D PAGE) and MALDI-TOF.
Results:
The proteins which included zinc finger protein, tumor necrosis factor receptor, and protein tyrosine phosphatase receptor were involved in gene transcription and signal transduction associated with cancer.
Conclusion:
NOR(1) gene may have some effects on liver cancer by up-regulating the expression of these proteins.
Related Concept Videos
Cell Specific Gene Expression
Cell Specific Gene Expression
Reporter Genes
Commonly used reporter...
What is Gene Expression?

