Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Nitric Oxide Signaling Pathway

Nitric Oxide Signaling Pathway

Nitric oxide (NO), an inorganic gas, acts as a potent second messenger in most animal and plant tissues. NO diffuses out of the cells that produce it and enters the neighboring cells to generate a downstream response. NO synthase (NOS) catalyzes NO production by the deamination of the amino acid arginine. There are three isoforms of NOS. Endothelial cells have endothelial NOS (eNOS), nerve and muscle cells have neuronal NOS (nNOS), and macrophages produce inducible NOS (iNOS) upon exposure to...

Potentiometry: Membrane Electrodes

Potentiometry: Membrane Electrodes

Membrane electrodes, also known as p-ion electrodes, use membranes that selectively interact with free analyte ions, generating a potential difference across the membrane. The resulting membrane potential, known as the asymmetry potential, is not zero even when analyte concentrations on both sides of the membrane are equal. The membrane's response is typically not selective to a single analyte but proportional to the concentration of all ions in the sample solution capable of interacting at the...

Types of Reversible Electrodes

Types of Reversible Electrodes

For electrode reversibility to be maintained, all the reactants and products involved in the half-reaction must be present at the electrode. There are several types of reversible electrodes (half-cells).In metal-metal-ion electrodes, a metal balances electrochemically with a solution of its own ions. Examples are Cu2+|Cu and Zn2+|Zn. Metals that react with the solvent, like group 1 and most group 2 metals, which react with water, and zinc, which reacts with aqueous acidic solutions, cannot be...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Micelle-Confined Co-N Coordination Enables Size-Tailored Nanoemitters for High-Performance ECL Biosensing.

Analytical chemistry·2025

Same author

Dual-Shell-Solidified Gold Nanoclusters-Based Electrochemiluminescence Sensing Platform for Highly Selective and Sensitive Detection of Cu<sup>2+</sup> and Histidine.

ACS applied materials & interfaces·2025

Same author

pH-gated bidirectional nanoassembly switches: self-ratiometric D-penicillamine sensing <i>via</i> AIE-active Au(I)-TCEP-Cd(II) coordination dynamics.

Analytical methods : advancing methods and applications·2025

Same author

Metal-π Interaction-Enhanced Electrochemiluminescence via U-Shaped Schiff Base Luminophores for Ultrasensitive Clindamycin Detection.

ACS sensors·2025

Same author

Metal-free photoinduced ROMP-enabled electrochemical aptasensor for ultrasensitive detection of endotoxin.

Talanta·2025

Same author

Janus Photothermal Comfortable Face Mask for Environmental Pollutant Monitoring.

ACS nano·2025

Search research articles

Related Experiment Video

Updated: Jul 7, 2026

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Published on: February 16, 2022

Nitric oxide selective electrodes.

Ian R Davies¹, Xueji Zhang

¹World Precision Instruments Limited, Aston, United Kingdom.

Methods in Enzymology

|February 2, 2008

Summary

This summary is machine-generated.

Electrochemical sensors offer a reliable method for measuring physiological levels of nitric oxide (NO) in real-time. This technique enables accurate in vitro and in vivo detection, advancing NO research.

More Related Videos

Analytical Techniques for Assaying Nitric Oxide Bioactivity

Analytical Techniques for Assaying Nitric Oxide Bioactivity

Published on: June 18, 2012

En Face Detection of Nitric Oxide and Superoxide in Endothelial Layer of Intact Arteries

En Face Detection of Nitric Oxide and Superoxide in Endothelial Layer of Intact Arteries

Published on: February 25, 2016

Related Experiment Videos

Last Updated: Jul 7, 2026

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Published on: February 16, 2022

Analytical Techniques for Assaying Nitric Oxide Bioactivity

Analytical Techniques for Assaying Nitric Oxide Bioactivity

Published on: June 18, 2012

En Face Detection of Nitric Oxide and Superoxide in Endothelial Layer of Intact Arteries

En Face Detection of Nitric Oxide and Superoxide in Endothelial Layer of Intact Arteries

Published on: February 25, 2016

Area of Science:

Biomedical Engineering
Analytical Chemistry
Physiology

Background:

Nitric oxide (NO) identified as endothelial-derived relaxing factor in the late 1980s.
Need for reliable methods to measure physiological NO levels.
Previous techniques had limitations in accuracy and real-time measurement.

Purpose of the Study:

To describe the development of electrochemical sensors for NO.
To detail sensor fabrication, detection principles, and performance characteristics.
To review experimental applications of NO selective electrodes.

Main Methods:

Fabrication of electrochemical sensors for NO detection.
Characterization of sensor performance: calibration, detection limits, selectivity, and response time.
Utilizing NO selective electrodes in various experimental settings.

Main Results:

Electrochemical method demonstrated reliability for measuring physiological NO levels.
Sensors provided accurate real-time measurements in vitro and in vivo.
Successful application of NO selective electrodes across diverse experimental studies.

Conclusions:

Electrochemical sensors are the definitive technique for physiological NO measurement.
The developed sensors offer high reliability, selectivity, and real-time capabilities.
These advancements facilitate further research into NO's biological roles.