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Updated: Jul 7, 2026

Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology
Published on: December 15, 2017
Applications of the VHb gene vgb for improved microbial fermentation processes
Xiao-Xing Wei1, Guo-Qiang Chen
1Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing, China.
Abstract:
Dissolved oxygen (DO) plays an important role in cell growth, especially in industry-scale microbial production. To alleviate the defects of hypoxic conditions, Vitreoscilla hemoglobin (VHb) has been used to enhance respiration and energy metabolism by promoting oxygen delivery. Heterologous expression of VHb in a variety of hosts has been shown to improve cell growth, protein synthesis, metabolite productivity, and bioremediation under oxygen-restricted conditions. In this chapter, many well-studied areas are presented to illustrate the potential of VHb application in microbial metabolic engineering industry. Also, applications of the vgb promoter have been discussed.
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