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Updated: Jul 7, 2026

Capillary Electrophoresis Separation of Monoclonal Antibody Isoforms Using a Neutral Capillary
Published on: January 16, 2017
Integration of various stacking processes in carrier ampholyte-based capillary electrophoresis
Jean-Marc Busnel1, Thomas Le Saux, Stéphanie Descroix
1Laboratoire Environnement et Chimie Analytique, UMR CNRS 7121, ESPCI, 10 rue Vauquelin, 75005 Paris, France. jean-marc.busnel@epfl.ch
Abstract:
Field-enhanced sample stacking, field-enhanced sample injection as well as electrokinetic supercharging have been successfully integrated in carrier ampholyte-based capillary electrophoresis. Through the analysis of different test sample mixtures, it has been shown that the carrier ampholyte-based background electrolytes, in spite of their very low conductivity, allow efficient online preconcentration of analytes by field-amplified techniques. Sensitivity enhancement factors of the same magnitude as those usually encountered with classical conductive background electrolytes have been obtained in such carrier ampholyte-based buffers. Depending on the online preconcentration method that has been integrated, sensitivity enhancement factors between 50 and several thousands have been reached.
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