Related Experiment Videos
[Preservation of hearts by perfusion under pressure]
F B Jatene1, M H Magalhães, P R De Oliveira
1Instituto do Coração do Hospital das Clínicas-FMUSP, São Paulo.
Purpose:
Development of method used to fix hearts in a reduced time, with preservation of their structures and characteristics.
Methods:
Fifty normal human hearts were fixed in a system with four reservoirs, a roller pump, PVC tubes and a plastic recipient. This system was used to maintain the continuous injection by pressure of formaldehyde solution, to the interior of the heart.
Results:
The fixation was satisfactory, pré-fixation time (13.93 +/- 5.13 min) and fixation time (60.53 +/- 12.50 min) were reduced related to previously utilized methods.
Conclusion:
The method was good, with decrease of fixation time and obtention of fixed hearts, with preservation of their structures and characteristics.