[Development of various multifunctional envelope-type nano device MEND based on novel assembly technologies]

Area of Science:

• Biotechnology
• Nanotechnology
• Molecular Biology

Context:

• Non-viral vectors face challenges like plasma, endosomal, and nuclear membrane barriers for effective gene delivery.
• Integrating multiple functional devices into a single delivery system is complex.
• Existing systems struggle with timed and localized function execution.

Purpose:

• To introduce a novel
• Programmed Packaging
• concept for non-viral gene delivery systems.
• To develop and evaluate a Multifunctional Envelope-type Nano Device (MEND) for delivering plasmid DNA (pDNA), oligodeoxynucleotides (ODN), and small interfering RNA (siRNA).

Summary:

• A Multifunctional Envelope-type Nano Device (MEND) was engineered based on the
• Programmed Packaging
• strategy.
• Octaarginine (R8)-modified MEND (R8-MEND) demonstrated high transfection efficiency, comparable to adenovirus, utilizing macropinocytosis to evade lysosomal degradation.
• R8-MEND successfully achieved in vivo gene delivery to mouse hair follicles via topical application.

Impact:

• The developed MEND system represents a significant advancement in non-viral gene delivery.
• This technology shows potential for therapeutic applications involving plasmid DNA and functional nucleic acids.
• The
• Programmed Packaging
• approach offers a versatile platform for designing sophisticated nanodelivery systems.