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Published on: July 31, 2019
Regulation of host cell cyclin D1 by Trypanosoma cruzi in myoblasts
Boumediene Bouzahzah1, Vyacheslav Yurchenko, Fnu Nagajyothi
1Department of Pathology, Albert Einstein College of Medicine, Bronx, New York 10461, USA.
Abstract:
Infection with the parasite Trypanosoma cruzi causes Chagas disease. In this study we demonstrated that there was an increase in cyclin D1 expression in T. cruzi (Tulahuen strain)-infected myoblasts. To examine a possible mechanism for the increased cyclin D1 expression we transfected L(6)E(9) myoblasts with cyclin D1 luciferase reporter constructs and infected with T. cruzi. There was no evidence of an increase in promoter activity. Additionally, quantitative PCR did not demonstrate any change in cyclin D1 message during infection. Moreover, we demonstrated that the cyclin D1 protein was significantly stabilized after infection. Collectively, these data indicate that infection with T. cruzi increases cyclin D1 protein abundance post-translationally.
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