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Updated: Jul 7, 2026
Lentiviral CRISPR/Cas9-Mediated Genome Editing for the Study of Hematopoietic Cells in Disease Models
Published on: October 3, 2019
Lentivirus transduction of bone marrow hemopoietic precursor cells with Lin-c-kit+ phenotype ex vivo using a genetic
N V Radyukhina1, P N Rutkevich, T I Aref'yeva
1Department of Cell Biology, Institute of Experimental Cardiology, Cardiology Research-and-Production Complex, Federal Agency for Health Care and Social Development, Moscow. cardiocell@cardio.ru
Abstract:
We developed a technology of labeling bone marrow precursor cells with the Lin-c-kit+ phenotype in culture by green fluorescent protein gene using a lentivirus vector. The proposed system provides effective transduction of bone marrow precursor cells and high transgene expression level in vitro (27%). The integration of the transgene into the transduced cell genome in vivo was verified by the method of splenic colonies.
