Related Experiment Video
Updated: Jul 7, 2026
Vein Interposition Model: A Suitable Model to Study Bypass Graft Patency
Published on: January 15, 2017
[Origin of neointimal cells in autologous vein graft in rat model]
Yong-Zhi Deng1, Su-Jian Liu, Li Ma
1Department of Cardiothoracic Surgery, the Second Affiliated Hospital of Shanxi Medical University, Taiyuan, China. dengyongzhi@hotmail.com
Objective:
To investigate the potential cell sources of neointimal cells in autologous vein graft in rat model.
Methods:
Vein graft neointimal cell origins were investigated using a model of vein-to-artery interposition modal. Slides were stained with hematoxylin and eosin, immunohistochemical staining was also performed with primary antibodies alpha-smooth actin or CD34.
Results:
Neointimal thickening was greater at the proximal ends (65.2 +/- 4.6) microm and, to a lesser extent, distal ends (64.7 +/- 5.3) microm, in comparison to the middle of the graft (63.5 +/- 5.6) microm. Vein-originating cells survived and make a contribution to neointimal formation within the vein graft, mostly adjacent to the lumen, suggesting an intimate association with endothelial cells, donor arterial smooth muscle cells or circulating progenitor cells.
Conclusions:
Vein graft neointimal cells arise predominantly from vein-derived endothelial cells, donor arteria smooth muscle cells or circulating progenitor cells. It suggests clinical relevance of stenosis-inhibiting therapies directed at the vein graft or early system pharmacologic administration.
More Related Videos
05:41Using a Cell-Tracer Injection to Investigate the Origin of Neointima-Forming Cells in a Rat Saccular Side Wall Model
Published on: March 16, 2022
07:02A Rabbit Venous Interposition Model Mimicking Revascularization Surgery using Vein Grafts to Assess Intimal Hyperplasia under Arterial Blood Pressure
Published on: May 15, 2020