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Methods for Performing Crosses in Setaria viridis, a New Model System for the Grasses
Published on: October 1, 2013
[Salt tolerance of Setaria verticillata L.: a short-cycle poaceae]
Hela Ben Ahmed1, Arafet Manaa, Ezzeddine Zid
1Unité d'écophysiologie et nutrition des plantes, département de Biologie, faculté des sciences de Tunis, université Tunis-El-Manar, campus universitaire, 1060 Tunis, Tunisie. benahmed_hela@yahoo.fr
Abstract:
The responses of growth, development, and nutrition to salt stress are examined in short-cycle Setaria verticillata. For these, two experiments are led. The first intended to study the effects of various concentrations of NaCl on the parameters of growth and nutrition during the vegetative phase. Fifteen-day-old platelets were grown on commercial peat irrigated with pure NaCl solutions (0 to 300 mM). After three weeks of culture, the plants were collected and divided into roots and shoots. The fresh and dry matter masses of the various bodies are given. The second experiment was intended to study the effect of different concentrations of NaCl on crop plants until maturity. The culture was led under the same conditions as the preceding one, but for three months until the end of the cycle (production and maturation of the seeds). At harvest, the plants were separated in roots, shoots, and grains. During all the development cycle, Setaria vertillata was very sensitive to salinity. The concentration of NaCl that caused an important reduction of dry weight production was about 75 mM. Dry matter deposition was more diminished in roots than shoots. The reduction of the production of growth observed seems associated with a higher accumulation of Na(+) in shoots and with a deficit alimentation of organs in K(+). During the reproductive phase, salt affects the components of the output and induces variability on the level of the production of biomass as significant as that noted during the phase of vegetative growth. Lastly, the capacity of germination of seeds was strongly dependent on the salt concentration of the culture medium of the plants mothers, a total loss of viability appearing on crop plants collected in the presence of NaCl 300 mM.
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