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Updated: Jul 7, 2026

Determination of Plasma Membrane Partitioning for Peripherally-associated Proteins
Published on: June 15, 2018
Phosphate availability affects the tonoplast localization of PLDzeta2, an Arabidopsis thaliana phospholipase D
Yoshiki Yamaryo1, Emmanuelle Dubots, Catherine Albrieux
1Laboratoire de Physiologie Cellulaire Végétale, CNRS, CEA, INRA, Université J. Fourier, CEA-Grenoble, iRTSV/LPCV, 17 Rue des Martyrs, Grenoble, France.
Abstract:
Under phosphate deprivation, higher plants change their lipid composition and recycle phosphate from phospholipids. A phospholipase D, PLDzeta2, is involved in this recycling and in other cellular functions related to plant development. We investigated the localization of Arabidopsis PLDzeta2 by cell fractionation and in vivo GFP confocal imaging. AtPLDzeta2 localizes to the tonoplast and the Nter regulatory domain is sufficient for its sorting. Under phosphate deprivation, AtPLDzeta2 remains located in the tonoplast but its distribution is uneven. We observed PLDzeta2-enriched tonoplast domains preferentially positioned close to mitochondria and beside chloroplasts. In absence of PLDzeta2, membrane developments were visualized inside vacuoles.
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