Related Experiment Videos
Essential differences in oncogene involvement between primary nodal and extranodal large cell lymphoma
S Raghoebier1, M H Kramer, J H van Krieken
1Department of Hematology, University Medical Center, Leiden, The Netherlands.
Blood
|November 15, 1991
Summary
This study investigated genetic alterations in large cell lymphomas (LCLs). Rearrangements of bcl-2 and c-myc oncogenes were linked to lymphoma origin and cell type, suggesting different genetic pathways for nodal versus extranodal LCLs.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Large cell lymphomas (LCLs) exhibit significant heterogeneity.
- Understanding the genetic basis of LCLs is crucial for diagnosis and treatment.
- B-cell type LCLs were the focus of this investigation.
Purpose of the Study:
- To investigate rearrangements of the bcl-2 and c-myc oncogenes in de novo B-cell LCLs.
- To correlate these genetic alterations with primary site, stage, and cytomorphology.
- To explore the hypothesis of different genetic origins for nodal and extranodal lymphomas.
Main Methods:
- Southern blot analysis was employed to detect gene rearrangements.
- Fifty-two cases of de novo B-cell LCLs were studied.
- Data were correlated with clinical presentation (primary site, stage) and cytomorphology.
Main Results:
- A t(14;18) translocation, indicated by bcl-2 rearrangements, was found in 40% of nodal lymphomas versus less than 5% of extranodal lymphomas.
- c-myc rearrangements were more frequent in extranodal lymphomas (35%) compared to nodal lymphomas (5%).
- bcl-2 rearrangements were associated with cleaved nuclei (50%), while c-myc rearrangements were linked to the noncleaved subtype (25%).
Conclusions:
- The study supports the hypothesis that primary nodal and extranodal lymphomas have distinct genetic origins.
- The presence and type of oncogene rearrangement correlate with the lymphoma's primary site and cytomorphology.
- These findings contribute to understanding the molecular pathogenesis of LCL subtypes.