High-performance liquid chromatography spectrometric analysis of tripterin in rat plasma

A quick, precise and reliable HPLC method has been developed to determine tripterin in rat plasma. After liquid-liquid extraction, the analytes was analyzed on a Discovery ODS C(18) column (5microm, 4.6mmx250mm) with an isocratic elution consisting of methanol-water-phosphoric acid (87:13:0.2, v/v/v). Ultraviolet detection was at 425nm. Using trioxymethylanthraquinone as an internal standard, the assay was linear over the concentration range of 0.025-1.60microg/mL (r(2)=0.9988). The extraction recovery of tripterin in rat plasma was more than 62%. The intra- and inter-day precision was less than 13% (CV). This validated method was successfully applied to the pharmacokinetics of tripterin in rats.