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Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

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Published on: February 16, 2022

The potential of Angeli's salt to decrease nitric oxide scavenging by plasma hemoglobin.

Xiaojun He¹, Ivan Azarov, Anne Jeffers

¹Department of Physics, Wake Forest University, Winston-Salem, NC 27109, USA.

Free Radical Biology & Medicine

|February 5, 2008

Summary

This summary is machine-generated.

Angeli's salt effectively treats intravascular hemolysis by converting cell-free hemoglobin to a less toxic form. This prevents nitric oxide scavenging, reducing pathological vasoconstriction in various diseases.

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Analytical Techniques for Assaying Nitric Oxide Bioactivity

Analytical Techniques for Assaying Nitric Oxide Bioactivity

Published on: June 18, 2012

Area of Science:

Biochemistry
Pathophysiology
Pharmacology

Background:

Intravascular hemolysis releases hemoglobin, contributing to disease pathology.
Cell-free hemoglobin scavenges nitric oxide, causing vasoconstriction.
Current treatments like inhaled nitric oxide have limitations.

Purpose of the Study:

To investigate compounds that selectively convert cell-free hemoglobin to less toxic species.
To evaluate Angeli's salt for treating intravascular hemolysis.

Main Methods:

Studied the reaction of nitroxyl from Angeli's salt with cell-free hemoglobin.
Compared reactions under physiologically relevant conditions.
Assessed hemoglobin oxidation state and nitric oxide scavenging potential.

Main Results:

Nitroxyl preferentially reacts with cell-free hemoglobin over encapsulated hemoglobin.
Oxygenated ferrous hemoglobin is oxidized to methemoglobin by nitroxyl.
Methemoglobin is converted to stable iron-nitrosyl hemoglobin.

Conclusions:

Angeli's salt effectively modifies cell-free hemoglobin, mitigating its toxicity.
This mechanism offers a potential therapeutic strategy for intravascular hemolysis.
Further development of Angeli's salt or similar compounds is warranted.