Previous maternal experience affects accumbal dopaminergic responses to pup-stimuli

The present study investigated the release of dopamine from the nucleus accumbens (shell) in response to pup-stimuli in the absence of lactation and maternal behaviors at time of sample collection. Subjects were female rats given maternal experiences through prior parturitions, recent pup-induced sensitization, or a combination of both. Nulliparous (N) or multiparous (M, had 2 prior litters but cycling) female rats either received pup-sensitization (S+) until they responded maternally in their homecage or no pup-sensitization (S-), thus, there were four groups: NS- (n=5), NS+ (n=6), MS- (n=5), and MS+ (n=8). Four hours after removal of pups (from homecage for S+ groups), all females were placed into the microdialysis chamber for sample collection. After baseline collection, four foster pups were given to the females. In this paradigm females show little to no maternal behavior in the test chamber. Samples (collected every 8 min) were analyzed for dopamine (DA) and 3,4-dihydroxyphenylacetic acid (DOPAC) with electrochemical detection using HPLC. Relative to the inexperienced NS- females, the experienced NS+, MS- and MS+ females displayed significantly increased DA levels only during the first 8 min of pup-exposure. The more experience a female had with pups, the greater was the DA response (p<.05). The results suggest that enhanced responding to pups following previous maternal experiences may be mediated through accumbal DA.