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In vitro Mutagenesis

In vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

In-vitro Mutagenesis

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

Mutagenicity and Carcinogenicity

Mutagenicity and Carcinogenicity

Mutagenicity and carcinogenicity refer to the ability of drugs to cause genetic defects and induce cancer, respectively. The International Agency for Research on Cancer (IARC) classifies agents into four groups based on their carcinogenic potential. Group 1 agents are known human carcinogens; group 2A agents are probably carcinogenic to humans; group 3 agents lack data to support their role in carcinogenesis; and group 4 includes agents for which data support that they are not likely to be...

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Related Experiment Video

Updated: Jul 7, 2026

Rapid Identification of Chemical Genetic Interactions in Saccharomyces cerevisiae

Rapid Identification of Chemical Genetic Interactions in Saccharomyces cerevisiae

Published on: April 5, 2015

A novel yeast-based tool to detect mutagenic and recombinogenic effects simultaneously.

B Schafer¹, A Neffgen, U Klinner

¹Department of Biology IV (Microbiology & Genetics), RWTH Aachen University, Worringer Weg, D-52056 Aachen, Germany. bernd.schaefer@rwth-aachen.de <bernd.schaefer@rwth-aachen.de>

Mutation Research

|February 5, 2008

Summary

A new yeast assay efficiently detects genotoxicity by monitoring mutations, recombination, and chromosome loss. This Saccharomyces cerevisiae strain offers a sensitive and reproducible method for assessing DNA-damaging agents, including aniline.

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The Lambda Select cII Mutation Detection System

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Last Updated: Jul 7, 2026

Rapid Identification of Chemical Genetic Interactions in Saccharomyces cerevisiae

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Published on: April 5, 2015

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The Lambda Select cII Mutation Detection System

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Published on: April 26, 2018

Area of Science:

Genetics
Molecular Biology
Toxicology

Background:

Genotoxicity testing is crucial for evaluating DNA-damaging agents.
Existing assays have limitations in detecting a broad spectrum of genotoxic events.

Purpose of the Study:

To develop a novel yeast-based assay for comprehensive genotoxicity detection.
To enable simultaneous monitoring of mutations, recombination, and chromosome loss.

Main Methods:

Construction of a diploid Saccharomyces cerevisiae strain with a URA3-kanMX4 DNA module.
Selection of genotoxicity events on 5-fluoroorotic acid (5-FOA) agar plates.
Phenotypic analysis of 5-FOA-resistant clones to detail genetic alterations.

Main Results:

The assay successfully detected genotoxic effects of UV radiation, N-methyl-N'-nitro-N-nitrosoguanidine, aniline, and benomyl.
Different agents induced mutations and recombination at varying frequencies.
The integrated module created a mutation/recombination hotspot, enhancing sensitivity.

Conclusions:

The developed yeast assay provides an easy, reproducible, and sensitive method for comprehensive genotoxicity assessment.
This system is capable of detecting mutagenic effects missed by other assays, such as those from aniline.