Related Experiment Video
Updated: Jul 7, 2026

Denaturing Urea Polyacrylamide Gel Electrophoresis (Urea PAGE)
Published on: October 29, 2009
[A simple, rapid and high-resolution banding method in polyacrylamide gels]
1Univesity of Electronic Science and Technology of China Zhongshan Institude, Guangdong, Zhongshan 528402, China. class2007ok@163.com
Abstract:
Although high-resolution picture can be obtained using the conventional banding method of DNA in PAGE gel, this method has complicated steps and will take very long time. In this paper, we reported an improved banding method of DNA in PAGE gel. DNA development in PAGE gel and comparison were performed by using two methods, the conventional banding method and the improved banding method, respectively. And the results indicated that the improved method has higher contrast between DNA band and the background in PAGE gel. So this improved method has higher resolution than the conventional one. Compared with the conventional banding method, this improved method has fewer steps, less time took and fewer reagents used. This improved method has replaced the conventional banding method completely in our laboratory.
More Related Videos
12:03Blue Native Polyacrylamide Gel Electrophoresis (BN-PAGE) for Analysis of Multiprotein Complexes from Cellular Lysates
Published on: February 24, 2011
10:27A Fast Silver Staining Protocol Enabling Simple and Efficient Detection of SSR Markers using a Non-denaturing Polyacrylamide Gel
Published on: April 20, 2018
Related Concept Videos
SDS-PAGE
A variation of gel electrophoresis, termed polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact proteins...
Two-dimensional Gel Electrophoresis
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as cells...
DNA Agarose Gel Electrophoresis
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...