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Replicative Cell Senescence

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Replicative cell senescence is a property of cells that allows them to divide a finite number of times throughout the organism's lifespan while preventing excessive proliferation. Replicative senescence is associated with the gradual loss of the telomere — short, repetitive DNA sequences found at the end of the chromosomes. Telomeres are bound by a group of proteins to form a protective cap on the ends of chromosomes. Embryonic stem cells express telomerase — an enzyme that adds the telomeric...

Replicative Cell Senescence

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Regulation of Expression at Multiple Steps

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Regulation of Expression Occurs at Multiple Steps

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Updated: Jul 7, 2026

A Suppressor Screen for the Characterization of Genetic Links Regulating Chronological Lifespan in Saccharomyces cerevisiae

A Suppressor Screen for the Characterization of Genetic Links Regulating Chronological Lifespan in Saccharomyces cerevisiae

Published on: September 17, 2020

Pathways change in expression during replicative aging in Saccharomyces cerevisiae.

Gloria Yiu¹, Alejandra McCord, Alison Wise

¹Biology Department, Pomona College, Claremont, CA 91711, USA.

The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences

|February 5, 2008

Summary

Yeast cells exhibit gene expression changes during aging, mirroring mammalian aging processes. Key findings include altered metabolism, reduced ribosome production, and increased DNA repair, offering insights into aging mechanisms.

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Last Updated: Jul 7, 2026

A Suppressor Screen for the Characterization of Genetic Links Regulating Chronological Lifespan in Saccharomyces cerevisiae

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Published on: September 17, 2020

Studying Age-dependent Genomic Instability using the S. cerevisiae Chronological Lifespan Model

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Published on: September 29, 2011

Continuous High-resolution Microscopic Observation of Replicative Aging in Budding Yeast

Continuous High-resolution Microscopic Observation of Replicative Aging in Budding Yeast

Published on: August 20, 2013

Area of Science:

Cellular Biology
Genetics
Molecular Biology

Background:

Yeast replicative aging shares similarities with mammalian cellular aging.
Phenotypic changes in aging yeast include cell enlargement, sterility, and nucleolar alterations.

Purpose of the Study:

To investigate gene expression alterations during yeast replicative aging.
To correlate observed gene expression changes with known aging mechanisms in yeast and higher organisms.

Main Methods:

Microarray analysis to study global gene expression.
Quantitative real-time reverse-transcription polymerase chain reaction (qPCR) to validate mRNA changes.

Main Results:

Identified significant messenger RNA (mRNA) changes at 12 and 18-20 generations.
Observed shifts towards aerobic metabolism, decreased ribosome gene expression, and partial environmental stress response.
Noted pseudostationary phase, downregulated methylation metabolism, increased nucleotide excision repair mRNA, and upregulated protein phosphatase I (Glc7) regulatory subunits.

Conclusions:

Yeast aging involves significant, reproducible gene expression changes.
These changes reflect metabolic shifts, stress responses, and DNA repair mechanisms.
Findings support the role of protein phosphorylation in yeast aging and its parallels with aging in higher eukaryotes.