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Updated: Jul 7, 2026
Heterotopic Auxiliary Rat Liver Transplantation With Flow-regulated Portal Vein Arterialization in Acute Hepatic Failure
Published on: September 13, 2014
[Experimental study of portal hemodynamics after microcapsule transplantation by intraportal and transarterial
Feng Gao1, Wei Wang, Rui-zhen Li
1Center of Cell Transplantation and Gene Therapy, Third Xiangya Hospital, Central South University, Changsha 410013, China.
Objective:
To compare the changes of portal hemodynamics after microcapsule transplantation by intraportal and transarterial approach with color doppler sonography.
Methods:
Thirty male dogs were divided into 2 groups randomly: Group PV (microcapsules implanted into the portal vein) and Group HA (microcapsules implanted into the hepatic artery). Both groups were divided into 3 subgroups(PV1, PV2, and PV3; HA1, HA2, and HA3) according to the number of implanted microcapsules (8,000/kg, 16,000/kg, and 32,000/kg). The internal diameter and flow velocity of the portal vein were measured with color doppler sonography before the transplantation,within 24 hours and 1, 2, and 4 weeks after microcapsule transplantation.
Results:
The flow velocity of the portal vein in Group PV1 and PV2 within 24 hours increased significantly (P<0.05); The meanwhile the internal diameters did not change significantly (P> 0.05). The flow velocity of the portal vein in Group PV3 within 24 hours decreased significantly (P<0.05), but the internal diameter increased significantly (P<0.05). Portal hemodynamics of different transarterial subgroups did not change significantly (P> 0.05). The level of ALT in Group PV and Group HA all increased significantly (P<0.05). And when the numbers of microcapsule transplantation were same, the level of ALT in subgroup PV were significantly higher than that in subgroup HA (P<0.05).
Conclusion:
Microcapsule transplantation by transarterial approach is safer than by intraportal way, and the hepatic artery can contain more microcapsule than the portal vein.
Insights
Transarterial microcapsule transplantation is safer than intraportal, with the hepatic artery accommodating more microcapsules. Intraportal transplantation showed significant portal vein flow changes and higher ALT levels, indicating potential risks.
Area of Science:
- Hepatology
- Vascular Surgery
- Medical Imaging
Background:
- Microcapsule transplantation is a novel therapeutic approach.
- Understanding the impact of different transplantation routes on portal hemodynamics is crucial for safety and efficacy.
Purpose of the Study:
- To compare portal hemodynamics following microcapsule transplantation via intraportal versus transarterial routes.
- To evaluate the safety and capacity of the hepatic artery versus the portal vein for microcapsule delivery.
Main Methods:
- Thirty male dogs were randomized into portal vein (PV) and hepatic artery (HA) groups.
- Microcapsule doses (8,000/kg, 16,000/kg, 32,000/kg) were administered.
- Portal vein diameter and flow velocity were assessed using color Doppler sonography at multiple time points.
Main Results:
- Intraportal transplantation (PV groups) showed significant portal vein flow velocity changes and increased ALT levels, especially with higher microcapsule doses.
- Transarterial transplantation (HA groups) resulted in no significant changes in portal hemodynamics.
- ALT levels were significantly higher in the PV group compared to the HA group for equivalent microcapsule doses.
Conclusions:
- Transarterial microcapsule transplantation is a safer approach than intraportal transplantation.
- The hepatic artery demonstrates a greater capacity for microcapsule containment compared to the portal vein.
