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Published on: July 27, 2016
[Expression and purification of human apolipoprotein M]
Min Hu1, Shui-ping Zhao, Tao Zhang
1Department of Cardiovasology, Second Xiangya Hospital, Central South University, Changsha 410011,China.
Summary
Researchers successfully cloned the human apolipoprotein M (ApoM) gene and expressed recombinant ApoM protein. This advancement facilitates further study of ApoM
Area of Science:
- Molecular Biology
- Protein Expression
- Biochemistry
Background:
- Apolipoprotein M (ApoM) plays a role in lipid metabolism and has potential implications in cardiovascular health.
- Understanding ApoM function requires a reliable source of the protein for experimental studies.
Purpose of the Study:
- To express and purify the full-length extracellular human apolipoprotein M (ApoM).
- To establish a method for producing recombinant ApoM for further research.
Main Methods:
- Polymerase Chain Reaction (PCR) amplification of the ApoM gene from a human liver cDNA library.
- Cloning of the ApoM gene fragment into the pGEXT vector for sequencing and confirmation.
- Transformation of the confirmed ApoM cDNA into E. coli DL21(DE3) for protein expression induced by IPTG.
Main Results:
- Successful amplification and cloning of the ApoM gene, confirmed by agarose electrophoresis (560 bp fragment).
- Sequencing verified the cloned gene's identity with the human ApoM gene in GenBank.
- SDS-PAGE analysis confirmed the expression of a 24 kD recombinant ApoM protein.
Conclusions:
- The human ApoM gene has been successfully cloned.
- Recombinant human ApoM protein has been expressed in E. coli.
